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[Preprint]. 2022 Dec 16:2022.12.16.520799. [Version 1] doi: 10.1101/2022.12.16.520799

Figure 4. MR-CoV-S6p312 elicits a Th1-oriented humoral and cellular immune response.

Figure 4.

(A) Isotype analysis of anti-SARS-CoV-2 spike antibodies. Serum samples from IFNAR−/−-CD46Ge mice vaccinated once (day 21) or twice (day 42) with MR-CoV-S6p312 were analyzed by ELISA for IgG1 and IgG2a antibody binding to SARS-CoV-2. Serum from mice vaccinated twice with purified SARS-CoV-2 Spike adjuvanted with alum was used as a control for the Th2-biased humoral response. (B) Cytokine production from the splenocytes of vaccinated mice. Splenocytes isolated from vaccinated mice were stimulated as indicated in Figure 1, and cytokine secretion in the supernatant was analyzed by multiplex cytokine analysis. Dots represent individual animals, and horizontal bars and error bars are the mean ± SD. IL-1b lower limit of detection (LLOD): 1.45 pg/mL; IL-12 LLoD: 1.68 pg/mL; TNF-a LLoD 3.48 pg/mL; IFN-γ LLoD 2.19 pg/mL; GM-CSF LLoD: 3.20 pg/mL;IL-6 LLoD: 5.52 pg/mL; IL-5 LLoD: 2.19 pg/mL; IL-2 LLoD 1.88 pg/mL;IL-4 LLoD: 1.37 pg/mL; IL-13 LLoD: 2.86 pg/mL. Statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparison test (*, p<0.05; **, p<0.003; ***, p<0.0003; ****, p<0.0001).