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Purification and reconstitution of YgaV: (A) SDS-PAGE analysis of purified His-tagged YgaV. Cell extracts of E. coli expressing N-terminally His-tagged YgaV, before and after IPTG induction, are also analyzed. Proteins were stained with Coomassie brilliant blue; (B) Absorption spectra of hemin, heme-reconstituted YgaV (heme-YgaV), purified YgaV before reconstitution (apo-YgaV), and 5-times concentrated purified YgaV before reconstitution (apo-YgaV, 5×).
