Figure 4.

Mobility shifts of YgaV caused by thiol modification on SDS-PAGE gels: (A) The purified apo-YgaV was treated with 1 mM DTT, 0.5 mM CuCl₂, 1 mM GSSG, and/or 10 mM Na₂S. The proteins were precipitated by trichloroacetic acid (TCA) treatment, labeled with AMS, and then applied for SDS-PAGE; (B) The AMS modification was performed with apo- and heme-reconstituted YgaV with different concentrations of Na₂S. The apo-YgaV was also treated with 0.5 mM CuCl₂ as a control. Proteins were stained with Coomassie brilliant blue.