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. 2022 Nov 30;11(12):2376. doi: 10.3390/antiox11122376

Figure 5.

Figure 5

rCT-NAMPT targets the inflamed colon. (A) Schematic illustration of rCT-NAMPT (upper). Bacterially purified 6xHis-rCT-NAMPT, rVehicle or rCT were analyzed by Coomassie Blue staining or IB with αHis (lower, left). BMDMs were treated with rVehicle, rCT, or rCT-NAMPT for the periods and concentrations stated, and cell viability was determined using the MTT test (lower, right). (B) DSS-induced model for colitis. Mice were subjected to 3% DSS for 6 days and were evaluated at day 6 (left). Colon harvests were used for IB with αITGA6, αITGB1 or αActin and analysis of the number of ITGA6+ or ITGB1+ cells by FACS. (C) The scheme of the acute colitis model transduced with Lenti-shNS or Lenti-shITG virus and treated with 3% DSS. Intraperitoneal administration of rCT-NAMPT conjugated with Cy5.5 for 1 h and colon harvest (left). Colon harvests were used for IB with αITGA6, αITGB1 or αActin and analysis of the number of ITGA6+ or ITGB1+ cells was done by FACS. The data come from three independent experiments that yielded comparable results (A–C).