Figure 6.

rCT-NAMPT has a medicinal effect against acute DSS-induced colitis in mice. (A) Scheme of the acute model of colitis transduced with Lenti-shNS or Lenti-shITG virus and subjected to 3% DSS with rCT-NAMPT (50 μg/kg) (upper). The survival of mice was monitored for 12 days; mortality was measured for n = 15 mice per group (lower). The statistical differences between the rVehicle-treated animals are noted (log-rank test). The data come from two different experiments that yielded comparable results. (B) Weight loss (n = 8). (C) Colitis scores were obtained from clinical parameters (weight loss, stool consistency, bleeding) (n = 8). (D) Image (upper) and length (lower) of colon in 3% DSS-treated mice with rVehicle, rCT or rCT-NAMPT (n = 8). (E) Colon was used for IP with αHis or αNAMPT, followed by IB with αTLR4, αCYBB or αCYBA. WCLs were used for IB with αTLR4, αCYBB, αCYBA, αHis or αActin. (F) FACS analysis for cellular ROS from colon (n = 8). (G) Levels of cytokines and MPO activity in colon homogenates (n = 10). (H) Hematoxylin and eosin (H&E) staining of the colon (left) (n = 10): representative imaging Histopathology scores were assessed in 3% DSS-treated mice with rVehicle, rCT, or rCT-NAMPT using H&E staining, as indicated in techniques (Materials and Methods). Statistical significance was assessed using the Student’s t-test with the Bonferroni correction (*** p < 0.001) in comparison to rVehicle.