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. 2022 Dec 14;9(12):805. doi: 10.3390/bioengineering9120805

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Study design. Three different adipose tissue-derived mesenchymal stromal cell (MSC(AT)) donors were expanded in fetal bovine serum (FBS) or human platelet lysate (HPL)-supplemented expansion medium in standard tissue culture plastic. MSC(AT) were encapsulated in alginate beads and kept at temperatures between 10 and 20 °C after reaching desired temperatures. MSC(AT) were left encapsulated during three different time periods: 30 min (D0), 5 days (D5), and 12 days (D12). Cells were then released and subjected to different characterization assays and compared with non-encapsulated MSC(AT). Cell retainment and survival during encapsulation, MSC identity and functional immunophenotype, MSC tri-lineage differentiation potential, metabolic activity, and hematopoietic support capacity were determined and compared between timepoints.