Figure 2.

Cell encapsulation performance and MSC(AT) metabolic analysis. (A) Cell recovery from alginate beads after 30 min (D0), 5 days (D5), and 12 days (D12) for MSC-FBS (blue) and MSC-HPL (red). (B) Cell viability of MSC(AT) before encapsulation (Non) and after their release from encapsulation at D0, D5, and D12. (C) Glucose and lactate concentration profiles. (D) Glucose (left) and lactate (right) profile regression modelling, including the fitting of first-order regressions with the presentation of the equation and coefficient of determination (R²). (E) Molar glucose consumption and lactate production rates. (F) Specific molar glucose consumption and lactate production rates at the various encapsulation timepoints. (Three MSC(AT) donors; mean ± SEM; * p < 0.05, ** p < 0.01.)