Figure 6.

Myoblast differentiation in differentiation medium (DMEM +1% ITS +1% P/S. (a) Immunofluorescence images of differentiated myoblasts (sarcomeric α-actinin staining) after 3 days of culture; (b) Ratio of differentiated cells relative to initial cell density; (c) Average number of nuclei inside myotubes; (d) Myotube density; (e) Myotube area; (f) Myotube diameter analysis of 80 random myotubes per condition; (g) Ratio of multinucleated myotubes. Graphs show mean ± standard deviation. (*), (**) and (***) indicate significant differences (p < 0.05, p < 0.01, and p < 0.001, respectively).