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. 2022 Dec 8;13:1066483. doi: 10.3389/fimmu.2022.1066483

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Copyright © 2022 Martín-Esteban, Rodriguez, Peske, Lopez de Castro, Shastri and Sadegh-Nasseri

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERAP1 activity is inhibited by octamers, not by heptamer: (A), ERAP1 was incubated with the indicated N-terminally extended peptides (left) and increasing concentration of octamer peptide inhibitor (Vertical; RLPSQYNF panel (A–E) or RYQKSTEL panel F–J), NI (No Inhibitor). (B), The N-terminally extended peptides were incubated with ERAP1 in the presence of increasing concentration of heptamer (YQKSTEL) panel (K–O). Trimming reactions were performed for 32 minutes at an E: S ratio of 1:10 and percent enzyme activity was calculated relative to the quantity of fully trimmed product in the NI reaction. Average and SD for each reaction were derived from three experiment. t-test analysis provided statistical significance for mean comparisons (P < 0.05). *** extremely significant, ** very significant and * significant.