Figure 3.

ERAP1 activity can rescue inhibition of ERAP2 R-AMC hydrolysis by long peptides (A); R-AMC hydrolysis time course [E:S, 1:30) (w/w), ERAP2 alone (Black), ERAP2 and R-AMC (Red), ERAP1 with R-AMC (Blue). AMC fluorescence was measured as a function of time. (B)] Increasing Long peptide concentrations were incubated with ERAP2 and R-AMC, IC50 for each peptide was calculated. (C); ERAP2 was incubated with R-AMC in the absence of peptide (Solid Red), or the IC50 for each long peptide (Dotted Blue). Long peptides were pre-incubated with ERAP1 for 32min, products were purified and added to a reaction containing ERAP2 and R-AMC (Dashed Green). The data are the mean of 3 experiments.