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. 2022 Dec 8;13:1066483. doi: 10.3389/fimmu.2022.1066483

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Copyright © 2022 Martín-Esteban, Rodriguez, Peske, Lopez de Castro, Shastri and Sadegh-Nasseri

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERAP2 activity is unaffected by octamer and shorter peptides, and ERAP1 long peptide digestion products (A); ERAP1 reaction products from previous long peptide substrates, digestions were performed for 32min. Recovery of long peptide digestion products longer than 9 amino acids (Red), 9-mers (Orange), or 8-mers (Yellow). The data are the mean of 3 experiments. (B); Time course of ERAP2 activity against R-AMC in presence or absence of an 11-mer (EIRRYQKSTEL), 8-mer(RYQKSTEL) and the 6-mer (QKSTEL).