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. 2022 Nov 28;11(12):1721. doi: 10.3390/biology11121721

Table 3.

Total reads number, clean reads number, Q20 value, Q30 value, total mapped reads, and unique mapped reads based on the RNA-Seq data in libraries of HXK and BXK. _1, _2, and_3 represent the three biological replicates of each sample. Q20 and Q30 represent the percentage of bases with a Phred value greater than 20 and 30, respectively.

Samples Total_Reads Clean_Reads %>Q20 %>Q30 Mapped Reads (%) Secondary Alignments (%) Unique Mapped (%)
BXK_1 81,727,748 81,727,706 97.81% 93.52% 67,113,867 (67.24) 18,086,546 (18.12) 49,027,321 (49.12)
BXK_2 93,641,990 93,641,948 97.87% 93.71% 76,691,067 (67.22) 20,445,351 (17.92) 56,245,716 (49.3)
BXK_3 106,129,474 106,129,422 97.96% 93.97% 86,499,777 (66.63) 23,691,204 (18.25) 62,808,573 (48.38)
HXK_1 91,740,416 91,740,386 97.92% 93.85% 77,487,325 (68.14) 21,970,557 (19.32) 55,516,768 (48.82)
HXK_2 96,117,694 96,117,654 97.73% 93.31% 81,692,006 (68.9) 22,455,670 (18.94) 59,236,336 (49.96)
HXK_3 94,944,072 94,944,020 97.78% 93.44% 81,438,637 (69.49) 22,253,903 (18.99) 59,184,734 (50.5)