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. 2022 Nov 26;11(12):2342. doi: 10.3390/antiox11122342

Figure 2.

Figure 2

Antibodies to hMPO, 2D10G9 (detects HOCl modified epitopes), and LYVE1 (glymphatic vessel marker) colocalize in dystrophic vessels bordering the lateral ventricles in the hMPO-A53T mouse brain. (A–C) Immunofluorescence staining of a whole mount sagittal section of hMPO-A53T mouse brain shows colocalization of 2D10G9 ((A), Alexafluor 594, red) and LYVE1 ((B), Alexafluor 488, green) and the merged image in Panel (C). Immunofluorescence is visible in vessels around the lateral ventricles (LV), and 4th ventricle under the cerebellum. The box (C) shows an example of location of vessels as seen in (D–F). Scale bar: (A–C) 1.5 mm. (D–F) Higher magnification (20×) of these regions shows the vessels around the ventricle stained for 2D10G9 ((D), Alexafluor 594, red) and LYVE1 glymphatic/lymphatic vessel marker ((E), Alexafluor 488, green) and the merged image (F). Scale bar: (D–F) 0.1 mm. (G–I) Higher magnification of panels of (D–F) (100×, oil immersion) shows the atypical structure of the vessels which have lumens surrounded by walls of filamentous and granular components. Some vessels appear to open to the lateral ventricle (LV). Scale bar: (G–I) 0.03 mm.