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. 2000 Dec;68(12):6526–6534. doi: 10.1128/iai.68.12.6526-6534.2000

FIG. 6.

FIG. 6

Additive effect of α-OS glucose and OpaA on MS11 invasion. Gentamicin protection assays were done using tissue culture medium containing either cytochalasin D (10 μg/ml) in DMSO or an equal volume of DMSO without cytochalasin D as a control (see text). Monolayers were infected in triplicate, and each experiment was done at least three times. Shown is one representative experiment each for Chang conjunctival (A) and ME-180 (B) cells. The graphs are plotted as triplicate values (dots) with means (horizontal lines). Data were analyzed by a modified Friedman statistical procedure (83, 84). The data in the left portion of each graph illustrate the additive effect of the proximal glucose and OpaA, in that MS11 lgtF (Opa) was consistently less invasive than either MS11 lgtF (OpaA+) in ME-180 cells or MS11 var C (Opa) in both Chang and ME-180 cells. The asterisk marks the value for invasion by MS11 lgtF (Opa) that reached statistical significance compared to MS11 var C (Opa). As expected, the invasion of OpaA+ GC was consistently inhibited by cytochalasin D; this is shown in the right portion of each graph. The plus signs denote significant inhibition of invasion by cytochalasin D.

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