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. 2022 Dec 14;11(12):1817. doi: 10.3390/antibiotics11121817

Table 1.

Primers used in the study.

Target Primer 5′-3′ Sequence Temperature (°C) Amplicon (bp) Reference
CTX-M CTX-M-F ATGTGCAGYACCAGTAARGTKATGGC 55 592 25
CTX-M-R TGGGTRAARTARGTSACCAGAAYSAGCGG
TEM TEM-F GCGGAACCCCTATTTG 50 963 26
TEM-R ACCAATGCTTAATCAGTGAG
SHV SHV-F AGCCGCTTGAGCAAATTAAAC 60 713 27
SHV-R ATCCCGCAGATAAATCACCAC
CTX group1 CTX group1-F TTAGGAARTGTGCCGCTGYA 52 688 27
CTX group1_2-R CGATATCGTTGGTGGTRCCAT
CTX group2 CTX group2-F CGTTAACGGCACGATGAC 52 404 27
CTX group1_2-R CGATATCGTTGGTGGTRCCAT
CTX group9 CTX group9-F TCAAGCCTGCCGATCTGGT 52 561 27
CTX group9-R TGATTCTCGCCGCTGAAG
CTX group8 CTX group8-F AACRCRCAGACGCTCTAC 52 326 27
CTX group8-R TCGAGCCGGAASGTGTYAT
CTX M-15 CTX M-15-F CACACGTGGAATTTAGGGACT 50 995 25
CTX M-15-R GCCGTCTAAGGCGATAAACA
gyrA gyrA-F AAATCTGCCCGTGTCGTTGGT 58 344 29
gyrA-R GCCATACCTACGGCGATACC
parC parC-F CTGAATGCCAGCGCCAAATT 57 168 29
parC-R GCGAACGATTTCGGATCGTC
qnrA qnrA-F ATTTCTCACGCCAGGATTTG 53 516 28
qnrA-R GATCGGCAAAGGTTAGGTCA
qnrB qnrB-F GATCGTGAAAGCCAGAAAGG 53 469 28
qnrB-R ACGATGCCTGGTAGTTGTCC
qnrS qnrS-F ACGACATTCGTCAACTGCAA 53 417 28
qnrS-R TAAATTGGCACCCTGTAGGC

Abbreviations: Bp: base pairs, F: forward, R: reverse.