Table 1.
Target | Primer | 5′-3′ Sequence | Temperature (°C) | Amplicon (bp) | Reference |
---|---|---|---|---|---|
CTX-M | CTX-M-F | ATGTGCAGYACCAGTAARGTKATGGC | 55 | 592 | 25 |
CTX-M-R | TGGGTRAARTARGTSACCAGAAYSAGCGG | ||||
TEM | TEM-F | GCGGAACCCCTATTTG | 50 | 963 | 26 |
TEM-R | ACCAATGCTTAATCAGTGAG | ||||
SHV | SHV-F | AGCCGCTTGAGCAAATTAAAC | 60 | 713 | 27 |
SHV-R | ATCCCGCAGATAAATCACCAC | ||||
CTX group1 | CTX group1-F | TTAGGAARTGTGCCGCTGYA | 52 | 688 | 27 |
CTX group1_2-R | CGATATCGTTGGTGGTRCCAT | ||||
CTX group2 | CTX group2-F | CGTTAACGGCACGATGAC | 52 | 404 | 27 |
CTX group1_2-R | CGATATCGTTGGTGGTRCCAT | ||||
CTX group9 | CTX group9-F | TCAAGCCTGCCGATCTGGT | 52 | 561 | 27 |
CTX group9-R | TGATTCTCGCCGCTGAAG | ||||
CTX group8 | CTX group8-F | AACRCRCAGACGCTCTAC | 52 | 326 | 27 |
CTX group8-R | TCGAGCCGGAASGTGTYAT | ||||
CTX M-15 | CTX M-15-F | CACACGTGGAATTTAGGGACT | 50 | 995 | 25 |
CTX M-15-R | GCCGTCTAAGGCGATAAACA | ||||
gyrA | gyrA-F | AAATCTGCCCGTGTCGTTGGT | 58 | 344 | 29 |
gyrA-R | GCCATACCTACGGCGATACC | ||||
parC | parC-F | CTGAATGCCAGCGCCAAATT | 57 | 168 | 29 |
parC-R | GCGAACGATTTCGGATCGTC | ||||
qnrA | qnrA-F | ATTTCTCACGCCAGGATTTG | 53 | 516 | 28 |
qnrA-R | GATCGGCAAAGGTTAGGTCA | ||||
qnrB | qnrB-F | GATCGTGAAAGCCAGAAAGG | 53 | 469 | 28 |
qnrB-R | ACGATGCCTGGTAGTTGTCC | ||||
qnrS | qnrS-F | ACGACATTCGTCAACTGCAA | 53 | 417 | 28 |
qnrS-R | TAAATTGGCACCCTGTAGGC |
Abbreviations: Bp: base pairs, F: forward, R: reverse.