Figure 4.

Knockdown of IKKα decreases NF-κB/IL-1β signaling in THP-1 cells. (A) Viability of H₂O₂ (0–30 μM)-treated THP-1 cells based on the WST-1 assay. (B) Levels of ROS measured by DCFH-DA staining. (C) mRNA levels of IL-1β in H₂O₂ (0–20 μM)-treated THP-1 cells. (D) mRNA level of IL-1β. THP-1 cells were transfected with siRNA-targeting IKKα (siIKKα) or scrambled sequence (siCtrl) for 12 h. BMS-345541 was used to block IKKα/β. The cells were then stimulated (or not) with H₂O₂ (5 μM) for 24 h. The cells were harvested for qRT-PCR assay or (E) immunoblotting. (F) Quantification of immunoblotting. Data are expressed as the mean ± SEM (n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control or 0 μM group. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. H₂O₂ group.