Boar semen contain a large bacterial community, including potential pathogens and putative antibiotic resistance genes. For this reason, different supplements have been evaluated that could contribute to decreasing the bacterial load in semen and maintaining quality parameters under freezing conditions and subsequent use in the industry. Within this group, we find natural molecules, antimicrobial peptides, and other supplements. Proline-arginine-rich antimicrobial peptides PR-39 (PR-39), porcine myeloid antimicrobial peptides 36 (PMAP-36), and 37 (PMAP-37), synthetic cyclic hexapeptides (c-WFW, c-WWW), pegylated single-walled carbon nanotubes (pSWCNTs), and magnetic silver nanocomposites (Ag-MNP). Created with BioRender.com.