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. 2022 Dec 10;11(12):1796. doi: 10.3390/antibiotics11121796

Table 2.

Studies on the use of antibiotics or alternative molecules in porcine sperm storage in recent years.

Authors Added Compound Recommended Concentrations
Antibiotics Bryla et al., 2015 [43] Gentamicin, florfenicol and
polymyxin B.
Combination of 100 μg/mL gentamicin and 100 μg/mL florfenicol
Schulze et al., 2017 [46] Gentamicin 512 mg/L
Feng et al., 2019 [44] Sulfanilamide. 0.02 g/L
Shaoyong et al., 2019 [49] ε-Polylysine 0.16 g/L
Luther et al., 2021 [45] Androstar® Premium, Beltsville
Thawing Solution, BTS®, Gentamicin,
Apramycin, Ampicillin
Gentamicin 0.1 mg/mL,
Apramycin 0.125 mg/mL and
Ampicillin 0.125 mg/mL
Tvrdá et al., 2021 [47] Gentamicin, aminoglycoside,
cephalosporin, lincomycin, spectinomycin
-
Natural Compounds Elmi et al., 2019 [50] Melaleuca alternifolia and
Rosmarinus officinalis
0.4 mg/mL
Shaoyong et al., 2019 [51] Kojic acid 0.04 g/L
Lustykova et al., 2020 [52] Gallic acid 0.008 mol/L
Manpoong et al., 2020 [53] Turmeric and KMnO4 Turmeric 0.5 mM and KMnO4 10 μM
Hensel et al., 2021 [54] Extracts from microalgae and hops 2 μg/mL
Lan et al., 2021 [55] Buckwheat honey 0.5%
Antimicrobial Peptides Schulze et al., 2014 [56] c-WFW and c-WWW 4 μM
Speck et al., 2014 [57] c-WWW, c-WFW, and MK5E c-WWW 2 μM, c-WFW 4 μM
and MK5E 1 μM
Bussalleu et al., 2017 [58] PR-39, PMAP-36, and PMAP-37 10 μM
Fang et al., 2017 [59] Iodine methionine 80 μM
Puig-Timonet et al., 2018 [60] PBD1 and PBD2 3 μM
Hensel et al., 2021 [54] C16-KKK-NH2 and C16-KKKK-NH2 8 μg/mL
Nanoparticles López-Pérez et al., 2017 [61] AgNPs 4 mM
Pérez-Durán et al., 2020 [62] AgNPs 20 mg/mL

PR-39: Proline-arginine-rich antimicrobial peptide 39, PMAP-36: Porcine myeloid antimicrobial peptides 36, PMAP-37: Porcine myeloid antimicrobial peptides 37, PBD1: Peptides porcine beta defensins-1, PBD2: Peptides porcine beta defensins-2, c-WFW: synthetic cyclic hexapeptide c-WFW, c-WWW: synthetic cyclic hexapeptide c-WWW, MK5E: Helical amide analog of magainin II, C16-KKK-NH2 and C16-KKK-NH2: Microbial lipopeptides, AgNPs: Silver nanoparticles, pSWCNTs: Pegylated single-walled carbon nanotubes, Ag-MNP: magnetic silver nanocomposites.