FIG. 7.

Effect of growth in DOC on localization of CDT proteins. Shown are Western blots of membrane fractions and 100-fold-concentrated supernatants of wild-type 81-176 cultured with or without DOC (25 mM) and immunodetected with ALM1 at a dilution of 1:10,000 (A), ALM2 at a dilution of 1:2,500 (B), and ALM3 at a dilution of 1:2,500 (C). Lane 1, 81-176, membrane fraction of cells grown without DOC; lane 2, 81-176, membrane fraction of cells grown with DOC; lane 3, 81-176, supernatant from cells grown without DOC; lane 4, 81-176 supernatant from cells grown in DOC. The amount of protein loaded was 2 μg per lane.