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Amoebicidal activity of polyhomoarginine (PHA) peptides against A. castellanii ATCC30868. In brief, 5 × 105 A. castellanii trophozoites were incubated with PHA peptides and chlorhexidine at 30 °C for 24 h after which viability was determined by staining with Trypan blue using a Neubauer haemocytometer. The results show significant anti-acanthamoeba activity when compared to amoeba+ PBS only (** p < 0.001 using one way ANOVA).
