Figure 3.

Inhibition of cysts formation by polyhomoarginine (PHA) peptides against A. castellanii ATCC30868. In brief, 5 × 10⁵ A. castellanii trophozoites were incubated with PHA peptides and chlorhexidine at 30 °C for 72 h after which cysts were determined by solubilizing trophozoites adding 0.25% SDS. The Number of cysts was counted using the Neubauer haemocytometer. The results show significant anti-acanthamoeba activity when compared to the amoeba + PBS with encystment medium (EM). (** p < 0.001, * p < 0.05 using one way ANOVA).