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Inhibition of trophozoites re-emergence from cysts by polyhomoarginine (PHA) against A. castellanii ATCC30868. In brief, 5 × 105 A. castellanii cysts were incubated with PHA peptides and chlorhexidine at 30 °C for 72 h after which trophozoites were determined by counting on a Neubauer haemocytometer. The results show significant anti-acanthamoeba activity when compared to the amoeba + PYG medium (** p < 0.001, * p < 0.05 using one way ANOVA).
