Figure 2.

Diagnosis of polymicrobial infections through metagenomics involves multiple steps, which can be broadly divided into three sections-(i) data generation that involves sampling, DNA isolation, and extraction, (ii) quality control, annotation, and normalization to avoid biases due to sequencing depth or batches, and (iii) analysis of the data in detection of pathogens, virulence factors, antibiotic resistance, and finding patterns.