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. 2000 Dec;68(12):6554–6560. doi: 10.1128/iai.68.12.6554-6560.2000

FIG. 4.

FIG. 4

Outer membrane protein profiles of B. cepacia K56-2 and K56orbA::tp electrophoresed on SDS–10% PAGE gel and stained with Coomassie brilliant blue. (A) Lane 1, molecular mass markers (kilodaltons) indicated on the left; lanes 2 to 6, 10 μg of outer membrane protein preparations; lane 2, K56-2 grown in medium with 20 μM EDDHA; lane 3, K56-2 grown in medium with 20 μM EDDHA and 10 μg of ornibactins/ml; lane 4, K56-2 grown in medium with 50 μM FeCl3; lane 5, K56orbA::tp grown in medium with no additions; lane 6, K56orbA::tp grown in medium with 50 μM FeCl3. (B) Complementation of K56orbA::tp with the orbA gene. Lane 1, molecular mass markers (kilodaltons) indicated on the left; lane 2, K56-2 grown in medium with 20 μM EDDHA; lane 3, K56orbA::tp(pUCP26); lane 4, K56orbA::tp(pPD526).