Figure 2.
Relative membrane potential of S. aureus cells expressed in fluorescence units of DioC2(3) reduced on an intact cell membrane resulting in green fluorescence. Cells were grown for 18 h in LB broth, washed with PBS, and resuspended until a final density of 106 CFU/mL in PBS supplemented with DioC2(3) to a final concentration of 10 μM/mL. After a 30 min incubation at 25 °C, compounds of interest were added to the samples. Fluorescence detection was performed for 30 min with 5 min intervals with excitation and emission wavelengths of 497 and 520 nm, respectively.