. 2000 Dec;68(12):6574–6579. doi: 10.1128/iai.68.12.6574-6579.2000

TABLE 2.

Effect of fimA promoter mutations (fimA_MP) on the expression of fimA::lacZ fusion genes

P. gingivalis strain	Promoter^a	Fusion	β-Galactosidase level^b
UPF	fimA_WT	fimA_WT::lacZ	260 ± 9
MP150A	fimA_MP150	fimA_WT::lacZ	158 ± 10
MPF35A	fimA_MPF35	fimA_WT::lacZ	17 ± 1
MPS10A	fimA_MPS10	fimA_WT::lacZ	300 ± 16

fimA_WT represents wild-type promoter; fimA_MP150 represents the fimA promoter with a 150-bp deletion of positive regulatory sequence spanning position −240 to −90 of the fimA gene; fimA_MPF35 represents the fimA promoter with a substitution mutation, from TTG to CCA, at position −46 to −44, affecting the −35 RNA polymerase binding site; fimA_MPS10 represents the fimA promoter with a substitution mutation, from TAA to GCC, at position −11 to −9, which does not affect promoter activity.

Miller units obtained from P. gingivalis grown at 37°C. Means ± standard deviations are indicated (n = 3).