Figure 3.
Inhibitory effect of geniposidic acid (GPA) on osteoclast differentiation of mouse BMMCs. BMMCs (2 × 105 cells/well) were cultured on 96-well plates in the presence of sRANKL (100 ng/mL) and M-CSF (20 ng/mL) in the presence or absence of GPA ((b): 0 μg/mL, (c): 100 μg/mL, (d): 1000 μg/mL) for seven days. (a) Osteoclasts with multiple nuclei (>3) in 96-well plates were counted (black arrowheads). The data represent the results of three independent experiments. (b–d) Representative TRAP-stained images of multinuclear osteoclasts in 96-well plates on day 7. Original magnification ×100; Scale bar = 100 µm. Results are shown as the mean ± SD. * Significantly larger number of osteoclasts by Student’s t-test (* p < 0.01).