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. 2022 Nov 30;11(12):1745. doi: 10.3390/biology11121745

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of inhibition of cardiac mtp and exercise training on cardiac function and Hand > mtp^RNAi whole-body TG levels in Drosophila. (A–I) Under different treatments, cardiac function assays included HR, HP, AI, DI, SI, DD, SD, FS and FL. NFD + w¹¹¹⁸, HFD + w¹¹¹⁸, HFD + E+w¹¹¹⁸, NFD + Hand > w¹¹¹⁸, HFD + Hand > w¹¹¹⁸, HFD + E + Hand > w¹¹¹⁸, NFD + Hand > mtp^RNAi, HFD + Hand > mtp^RNAi, and HFD + E + Hand > mtp^RNAi. Sample sizes N = 26, 28, 24, 27, 28, 19, 34, 22 and 22. All samples were virgin flies of 10 days. (J) Whole-body TG levels of Hand > mtp^RNAi Drosophila. All samples were virgin flies of 10 days, N = 10, and measurements were repeated three times. Whole-body TG levels were significantly higher in Drosophila in the HFD + E group compared to the HFD group. One-way ANOVA was used to identify differences between the strains of Drosophila NFD, HFD and HFD + E. LSD was used for post hoc testing.