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. 2022 Dec 15;10(12):3261. doi: 10.3390/biomedicines10123261

Figure 3.

Figure 3

Assessment of the CD4+ T and B cells responses specific to the Wuhan SARS-CoV-2 spike and the B 1.1.529 SARS-CoV-2 VOC spike, 2 months after the administration of the fourth vaccine dose in eight vaccinated PLWH vaccinated. (A) Visualization of the SARS-CoV-2 spike protein (1273 aa) covered by the S1, S+ and S peptide pools, the wild type sequence and the mutated sequence (aa 19–981) in the Omicron B1.1. 529 (BA.1). All peptide pools contain 15-mer peptides, 11 aa overlapping. (B) The frequencies of the CD154+ cells producing at least 1 cytokine among IL-2, IFN-γ and TNF-α, stimulated with the spike peptide pool, wild type (Wuhan) or the B. 1.1.529 (Omicron) peptide pool covering the mutated sequence of the spike. (C) Representative plots of the identification of the Wuhan spike-specific, labelled with APC-PB, and the Omicron spike-specific B cells, labelled with PE-PeVio770 within the Wuhan spike-specific pool. (D) Frequencies of the spike specific memory B cells binding both the Wuhan spike and Omicron spike and the B cells binding exclusively to the Wuhan spike. Each color corresponds to a different subject. Black lines indicate mean values.* p ≤ 0.05, ** p ≤ 0.005. Error bars indicate SE values.