Figure 10.

Suppression of FISH of the microdissected DNA probe of the abnormal chromosome (WCPinv10): (a) by metaphase chromosomes of a healthy individual (green signal) and (b) by metaphase chromosomes of the patient (red signal). Labels. 10: the normal homolog of chromosome 10; inv10: chromosome 10 containing the paracentric inversion in the long arm. Profiles of the intensity of the FISH signal and DAPI staining along chromosomes 10 and inv(10) are presented in the block. The intensity profiles of hybridization signals are determined relative to the line highlighted in red along chromosomes 10 and inv(10). Chromosomes were counterstained with DAPI (blue).