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. 2022 Dec 8;12(12):1837. doi: 10.3390/biom12121837

Figure 1.

Figure 1

Calibration of the biaxial stretch system. (A) Schematic of the sectioned PDMS membrane utilized for calibration. Each box (A–E) is 0.25 cm × 0.25 cm. (B) Membrane displacement after stretch at each of the five locations indicated in (A). (C) Fluorescent image demonstrating staining of actin (green) and DAPI counterstain (blue) after 24 h of static culture on a PDMS membrane at 43× magnification. (D) Fluorescent image demonstrating actin staining (green) and DAPI counterstain (blue) after 24 h of biaxial stretch at 43× magnification. Scale bar represents 20 µm.