Figure 2.

U937 cell gene expression levels measured by qRT-PCR. (A) Expression levels of CD11b in undifferentiated and macrophage-differentiated U937 cells. CD11b was significantly upregulated after differentiation. (B) Differentiated cells showed morphological changes, from suspension cells to attached, macrophage-like cells. Magnification 200×. (C) TNF-α and IL-1β expression increased after differentiation, while IL-6 and RETN expression decreased. RQ = relative quantification. * p ≤ 0.05, *** p ≤ 0.001. Data presented as means ± SEMs.