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PTx treatment of host cells does not affect the residual cell invasion capacity of OPBnull trypomastigotes. NRK fibroblasts were pretreated or not with 0.4 mg Ptx per ml overnight at 37°C. After treatment, the toxin was removed and the cells were exposed to WT or OPBnull trypomastigotes for 30 min. The data represent the average of triplicates ± the standard deviation (SD).
