TABLE 1.
N-terminal sequencing and mass spectroscopy of recombinant proteins
| Protein | Positiona | N-terminal amino acid sequenceb | Mol wt
|
|
|---|---|---|---|---|
| Predicted | Observed | |||
| Pvs25 | 1 | EAEASAVTVDTI | 20,524.2 | 20,500.6 |
| Pvs28 | 1 | EAE(A)(S)KV(T)A(E)T(Q) | 21,533.9 | 21,619.3 |
| 10 | ETQ(C)(K)(N)G(Y)V(V)Q(M) | 20,646.9 | 20,496.4 | |
| 12 | (Q)(C)KNGYV(V)(Q)(M)SN | 20,416.7 | 19,533.0 | |
a
Position of the first sequenced amino acid with reference to the predicted sequence of the mature secreted recombinant proteins.
b
Five amino acids (EAEAS) are derived from vector sequences. Amino acids in parentheses were not detected.