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. 2022 Dec 9;14(24):6062. doi: 10.3390/cancers14246062

Figure 5.

Figure 5

PRI-724 inhibits proliferation of Huh6 and HepG2 cells. (A) mRNA levels coding for cell cycle proteins by QRT-PCR. (B) Expression of cell cycle proteins determined by Western blot. (C) MTT cell proliferation assay with HepG2 (48 h, p = 0.0142; 72 h, p = 0.0258) and Huh6 (48 h, p = 0.0012; 72 h, p = 0.0025) cells treated with DMSO and PRI-724 (one-way ANOVA). (D) Images of cells at 72 h after addition of DMSO or PRI-724. Scale bar is 200μm. (E,F) Huh6 and HepG2 cells treated with DMSO or PRI-724 were examined by IF with antibodies to ph-S675-β-catenin and GPC3 and stained with DAPI. Arrows indicate mitotic figures. (G) Calculations of percentage of ph-S675-β-catenin-positive HepG2 and Huh6 with mitotic figures treated with DMSO or PRI-724. Three large (10×) fields were counted for each treated cell line (HepG2 p = 0.0219, Huh6 p = 0.0005, unpaired t-tests) (* p ≤ 0.05, *** p ≤ 0.001). The uncropped Western blots have been shown in Figure S9.