TABLE 1.
Strains and plasmids used
| Strain or plasmid | Relevant genotypea | Source or reference |
|---|---|---|
| Y. enterocolitica | ||
| JB580v | Serogroup O:8 Nalr ΔyenR (R− M+) | 29 |
| JB41v | JB580v wild-type inv; inv::phoA Nalr Camr | 1 |
| ASG21 | As JB580v with a TnMax2 insertion in sif15, Nalr Ermr | This study |
| E. coli | ||
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE phoA20 thi-1 rpsE rpoB argE(Am) recAl λ pir lysogen | 24 |
| S17-1λpir | Tpr StrrrecA thi pro hsdR hsdM+ RP4::2-Tc::Mu::Km Tn7 λ pir lysogen | 38 |
| Plasmids | ||
| pEP185.2 | MobRP4 oriR6K Camr | 29 |
| pFUSE | MobRP4 oriR6K Camr, polylinker upstream of promoterless lacZYA | 2 |
| pGY2 | MobRP4 oriR6K Apr Spcr Strr, unique BglII site upstream of promoterless cat | 54 |
| pHG329 | lacZα Apr, medium-copy-number cloning vector | 47 |
| pRK2013 | Tra+ Kmr | 40 |
a
Tp, trimethoprim; Str, streptomycin; Ap, ampicillin; Km, kanamycin.