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. 2022 Dec 12;10(12):3223. doi: 10.3390/biomedicines10123223

Figure 2.

Figure 2

Populations of major histocompatibility complex (MHC) IIhi CD11c+ cells and differentiation of interferon (IFN)-γ-expressing effector T cells in draining lymph nodes after corneal transplantation. Populations of MHC IIhi CD11c+ dendritic cells (DC) and differentiation of interferon (IFN)-γ-expressing effector T cells in draining lymph nodes (LNs). (A) Representative flow cytometry plot showing MHC IIhi CD11c+ cells after CD11c+ gating. (B) Representative flow cytometry plot presenting the differentiation of IFNγ+ CD4+ cells. (C) Quantitative analysis of MHC IIhi CD11c+ cell populations (n = 3). Comparison between MDSCs (MDSC iv, MDSC scj) and vehicle (PBSiv, PBSscj) showed that the MDSCs groups presented a decreased maturation of MHC IIhi CD11c+ cells, whereas the vehicle groups did not. The immunomodulating effect of MDSCs was significantly better in the local (scj) injection group than in the systemic (iv) injection group. (D) Quantitative analysis graph of IFNγ+ CD4+ cell differentiation (n = 3). MDSCs groups (MDSC iv, MDSC scj) led to inhibit differentiation of IFNγ+ CD4+ cells when compared with vehicle groups (PBSiv, PBSscj). Representative flow cytometric data from three independent trials with pooled cells from three mice per group. * p < 0.05, ** p < 0.01.