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. 2022 Nov 24;12(12):1749. doi: 10.3390/biom12121749

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Concentration-dependent effects of recombinant BG (rhBG) on cell viability and proliferation. Endometriotic epithelial 12Z cells were treated with increasing concentrations of the recombinant BG (5–200 ng/mL) and were analyzed for cell viability (A) and proliferation (B) after 24 h using the CCK-8 and BrdU assay kits, respectively. BG treatment had no effect on the number of viable cells and cell proliferation at the concentrations tested. Untreated cells were used as a control and the mean OD of the control was set to 100%. Each bar represents the mean ± SEM of 3 independent experiments performed in duplicate. Dunnett’s test was used for statistical analysis; rhBG, recombinant betaglycan.