FIG. 3.
Complementation of MSC57360 neuC1 in trans. Plasmid pMSC1420 was conjugally mobilized from E. coli DH5α (RK212.2) into MSC57360 neuC1. Proteinase K-digested whole-cell preparations were electrophoresed on 16% Tricine gels and silver stained (A), reacted with CT (final concentration, 1 μg/ml) (B) or immunodetected with polyclonal rabbit antiserum against whole cells of MSC57360 (final dilution, 1:500) (C). Lane 1, MSC57360; lane 2, MSC57360 neuC1; lane 3, MSC57360 neuC1 (pMSC1420).