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. 2000 Dec;68(12):6656–6662. doi: 10.1128/iai.68.12.6656-6662.2000

FIG. 3.

FIG. 3

Complementation of MSC57360 neuC1 in trans. Plasmid pMSC1420 was conjugally mobilized from E. coli DH5α (RK212.2) into MSC57360 neuC1. Proteinase K-digested whole-cell preparations were electrophoresed on 16% Tricine gels and silver stained (A), reacted with CT (final concentration, 1 μg/ml) (B) or immunodetected with polyclonal rabbit antiserum against whole cells of MSC57360 (final dilution, 1:500) (C). Lane 1, MSC57360; lane 2, MSC57360 neuC1; lane 3, MSC57360 neuC1 (pMSC1420).