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Using CRISPR-Cas9 system to create mutants. (A). Design of the sgRNA site for OsNAC092 exons. (B). osnac092 T0 genotype digestion test results. (C). Sanger sequencing results of osnac092 T0 generation. (D). MS + 150 mmmol mannitol germination results under simulated drought treatment. osnac092 (T/T) and osnac092 (A/A), insertion of a single base pair (+1 bp/+1 bp). osnac092 (−1/−1), a single base pair deletion (−1 bp/−1 bp).
