Table 4.
Comparison of SERS and LSPR-based nanobiosensors for viral RNA detection.
| Analytical Method | Feature | Target | Required Time | Detection Limit | Ref |
|---|---|---|---|---|---|
| Raman | Silver nanoparticles (Ag NPs) as Raman substrates with multivariate data analysis technique, PCA and PLSR | HCV RNA | - | 2.55 log IU/mL | [80] |
| SERS | PCR-integrated detection system, silver nanoparticles (Ag NPs) as SERS substrates with multivariate data analysis technique, PCA and PLS-DA | HBV and HCV RNA | - | - | [81] |
| SERS | SERS-active silver nanorods (AgNRs) sensing chips and a specially designed smart unlocking-mediated target recycling signal amplification | SARS-CoV-2 RNA | 50 min | 51.38 copies/mL | [82] |
| SERS | Gold nanostars functionalized with a Cy3-tagged beacon DNA for in vitro sensing | Hemagglutinin (HA) segment | - | - | [83] |
| LSPR | Two-dimensional gold nanoislands (AuNIs) functionalized with complementary DNA receptors | SARS-CoV-2 RNA | 800 s | 0.22 pM | [84] |
| LSPR | Gold and silver (Au–Ag) alloy nanoshells integrating LAMP method | SARS-CoV-2 RNA | 75 min | 10 copies/rxn | [85] |
| LSPR | Plasmonic AuNPs capped with suitably designed thiol-modified antisense oligonucleotides (ASOs) for colorimetric sensor | SARS-CoV-2 RNA | 10 min | 0.18 ng/μL | [86] |
| LSPR | Colorimetric/SERS/fluorescence triple-mode biosensor based on 17 nm-sized AuNPs | SARS-CoV-2 RNA | 40 min | 160 fM | [87] |