Figure 1.

(A) Chrysanthemum coronarium L. (CC) extract reduces the senescent phenotype in H₂O₂ (100 μmol/L)-stimulated HUVECs, as shown by β-gal staining. The magnification was set to 40×. Scale bar = 50 μm. (B) Quantitative analysis of positive SA-β-gal stained cells. Data shown are derived from three independent experiments and expressed as the mean ± SEM (n = 6 per group). (C) CC reduced the expression of p21, p53, and PAI-1 induced by H₂O₂. (D–F) The relative ratios from comparison to β-actin in duplicate blots are shown in a graph chart. Control cells (CB) received the vehicle alone. The T Data shown are derived from three independent experiments and expressed as the mean ± SEM (n = 6 per group). **, p < 0.01, ***, p < 0.001, versus CB; #, p < 0.05, ###, p < 0.001, vs. H₂O₂.