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The effect of 590 nm LED irradiation on cell viability and cell apoptosis of HMEC-1. (A) Cell viability of HMEC-1 measured using CCK-8 assay 12 h, 24 h and 48 h after 0–50 J/cm2 590 nm LED illumination. Results were normalized to the control. (B) Apoptosis of irradiated cells detected by flow cytometry (24 h, Annexin-V FITC & PI staining). Data are presented as means ± SD in independent experiments. n = 3; *, p < 0.05. n.s., no significance.
