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. 2022 Dec 16;11(24):4085. doi: 10.3390/cells11244085

Figure 9.

Figure 9

CAR-T cell proliferation in PB measured by qPCR. In the murinized group, (A) the median peak of circulating CD19 CAR-T cells, detected by qPCR, was 106,000 copies/μg genomic DNA (range: 5.07–2,090,000), occurring on day 10 (range: day 7–day 60); (B) the median peak for CD22 CAR-T cells was 12,850 copies/μg genomic DNA (range: 1–328,000), occurring on day 14 (range: day 7–day 30). (C) In the humanized group, the median peak of circulating CD19 CAR-T cells was 122,500 copies/μg genomic DNA (range: 18,600–280,000), occurring on day 12 (range: day 7–day 30); (D) the median peak of circulating CD22 CAR-T cells was 27,150 copies/μg genomic DNA (range: 1.17–136,000), occurring on day 14 (range: day 7–day 180). The comparison of the proliferation of the CAR19 cells (E) and CAR22 cells (F) in the murinized and humanized group. There was no significant difference between the CAR-T cell proliferation between the two groups, but the duration of the humanized CAR-T cells was longer than that of the murinized CAR-T cells. PB: peripheral blood; CAR-T: chimeric antigen receptor-T cell; qPCR: quantitative real-time polymerase chain reaction.