Skip to main content
. 2022 Nov 27;44(12):5902–5914. doi: 10.3390/cimb44120402

Figure 4.

Figure 4

Effects of Arecae Pericarpium ethanol extract on glutamate-induced apoptosis in HT22 cells. Cells were pretreated with APE at concentrations of 50, 100, or 200 μg/mL and then treated with glutamate (5 mM). (A) Apoptosis of HT22 cells was evaluated using flow cytometry. The image on the top right shows the percentage of healthy, early apoptotic, late apoptotic, and necrotic cells for each treatment group. (B) The expression levels of BAX, Bcl-2, PARP, and cleaved-PARP were determined by Western blot analysis. Control was non-treated cells. Blot images represent the three independent experiments. Data were presented as mean ± standard error of the mean. Statistical significance was set at # p < 0.05 (vs. control), * p < 0.05, ** p < 0.01, and † p < 0.001 (vs. glutamate). APE, Arecae Pericarpium ethanol extract; BAX, Bcl-2-associated X; Bcl-2, B-cell lymphoma 2; Con, control; Glu, glutamate; PI, propidium iodide; PARP, Poly(ADP-ribose) polymerase.