FIG. 2.

Primer extension analysis of total RNA from A. naeslundii WVU45 cells grown under conditions of nitrogen excess (lane 1, ADM/8 supplemented with 2% casamino acids; lane 2, ADM/8 supplemented with 2% tryptone) or nitrogen limitation (lane 3, ADM/8). The nucleotide sequence of the promoter region immediately 5′ to the transcription initiation site is indicated. DNA sequences that demonstrate significant homologies to consensus recognition sequences for RNA polymerase associated with ς⁷⁰ or ς⁵⁴ are underlined.