TABLE 2.
Urease activity in A. naeslundii WVU45 cells grown on various nitrogen sourcesa
| Medium | Urease sp act (nmol/mm/mg) | SD |
|---|---|---|
| ADM/8 (0.025% Casamino Acids) | 61.1 | 11.95 |
| ADM/4 (0.05% Casamino Acids) | 31.7 | 15.3 |
| ADM/8 + 25 mM ammonium chloride | 25.2 | 9.37 |
| ADM/8 + 50 mM ammonium chloride | 18.1 | 1.1 |
| ADM/8 + 25 mM asparagine | 32.0 | 7.15 |
| ADM/8 + 50 mM asparagine | 29.56 | 4.86 |
| ADM/8 + 25 mM glutamine | 23.49 | 10.58 |
| ADM/8 + 50 mM glutamine | 21.4 | 2.6 |
| ADM/8 + 25 mM urea | 40.6 | 10.48 |
| ADM/8 + 2% Casamino Acids | 11.3* | 5.99 |
| ADM/8 + 2% tryptone | ND† |
a
ND, none detected [<1.5 nmol of urea hydrolyzed min−1 (mg of cell dry weight)−1]. ∗, value differs significantly from that for cells cultivated in 0.025% Casamino Acids; † , value differs significantly from that for cells cultivated in 0.05% Casamino Acids.