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. 2022 Dec 15;14(24):6199. doi: 10.3390/cancers14246199

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Enrichment of the glioblastoma stem cell fraction. (A–D). Culturing murine SMA-560, SMA-540 and GL-261 glioma cells and human U-251MG glioma cells in NSC medium (open circles) increased housekeeper-normalized mRNA abundances of stem cell markers as compared to DMEM medium (closed circles; in addition to the individual values, means ± standard error of three experimental and two observational units each are depicted). The mRNA abundances of four different stem cell markers, (A) ALDH1A3, (B) CXCR4, (C) Nestin and (D) SOX2 were determined by real-time RT-PCR. Numbers indicate error probability (p values) as calculated by Welch-corrected two-tailed t-test.