Figure 6.
Eribulin induces molecular markers of DNA damage and apoptosis in acute leukemia cells. (A) Western blot analysis for levels of phospho(p)-STMN1S16, STMN1, γH2AX, and PARP1 (total and cleaved) in total cell extracts of vehicle- and eribulin-treated acute leukemia cells (0.25, 0.5, and 1 nM) for 48 h; Membranes were incubated with the indicated antibodies and developed with the SuperSignal West Dura Extended Duration Substrate System and images were acquired with a Gel Doc XR+. (B) Bar graphs represent the mean ± SD of three independent experiments quantifying the indicated protein band intensities. * p < 0.05, ** p < 0.01, *** p < 0.001; ANOVA and Bonferroni post-test.